Ion Exchange Column Chromatography

Ion exchange chromatography definition or ion chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger.

Ion exchange column chromatography.

Ion exclusion chromatography is defined 91 as a technique used to separate weak acids amino acids sugars alcohols and other substances on an ion exchange column. Because of donnan exclusion ionic material is excluded from the ion exchange resin and passes quickly through the column. Ion exchange chromatography is an interesting type of column chromatography. Ion exchange chromatography workflow.

Ion chromatography or ion exchange chromatography separates ions and polar molecules based on their affinity to the ion exchanger. For interaction to occur the protein of interest must have a charge opposite to that of the functional group of the sorbent particle. As you know the chromatography is a process of the separation of molecules from a mixture. Ion exchange chromatography which is designed specifically for the separation of differently charged or ionizable compounds comprises from mobile and stationary phases similar to other forms of column based liquid chromatography techniques 9 11.

It can be used for almost any kind of charged molecule including large proteins small nucleotides and amino acids. Key steps in the ion exchange chromatography procedure are listed below. It works on almost any kind of charged molecule including large proteins small nucleotides and amino acids however ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein. This separation is done based on the differences in the adsorption coefficient or partition coefficient of the sample with the stationary phase.

After loading an impure protein sample onto an ion exchange chromatography column the column is washed to remove undesired proteins and other impurities and then the protein s of interest is eluted using either a salt gradient or a change in ph. It is usually used for protein purification but may be used for purification of oligonucleotides peptides or other charged molecules.