They are well suited for processing feedwater for medium pressure boilers and for process water for the beverage industry.
Ion exchange column diagram.
Ion exchange usually describes a processes of purification of aqueous solutions using solid polymeric ion exchange resin more precisely the term encompasses a large variety of processes where ions are exchanged between two electrolytes.
For example metal contaminants are undesirable due to their color taste odor or toxicity.
Key steps in the ion exchange chromatography procedure are listed below.
Ion exchange takes place in one column while the resin in the second column is being regenerated resulting in continuous operation.
Ion exchange chromatography can be applied to the determination of ionic solutes such as inorganic anions cations transition metals and low molecular weight organic acids.
Cation exchange chromatography workflow.
Schematic diagram of the principles of chromatography as discovered by tswett 1901.
A separation column and a detector to measure the species eluted from the column.
The ion exchange column has a solid resin feed of 10 m 3 h and an interstitial liquid factor of 0 85.
The interstitial liquid fed with the resin contains caustic soda and the liquor flow corresponds to one bed volume.
Split stream and weak acid cation systems also reduce the total dissolved solids.
Usage examples continuous ion exchangers are used when it is imperative that product be continually generated.
Ion exchange chromatography workflow.
While the chemistry of individual ion exchange reactions varies from one application to the next ix is a treatment process where dissolved ions are replaced by other more desirable ions of a similar electrical charge.
The hardness ions ca and mg move into the resin beads and each of these divalent.
The table below shows the material flows in m 3 h around the ion exchange column.
All ion exchange chromatography relies on electrostatic interactions between the resin functional groups and proteins of interest.
Ion exchange ix systems are used across a variety of industries for water softening purification and separation purposes.
After loading an impure protein sample onto an ion exchange chromatography column the column is washed to remove undesired proteins and other impurities and then the protein s of interest is eluted using either a salt gradient or a change in ph.
An impure protein sample is loaded into the ion exchange chromatography column at a particular ph.
Thus the workflow below is given as a generalized iex workflow and particular running conditions for cation exchange chromatography may be adjusted to best suit your protein of interest the buffer system and the anion.
